pCSD_N-SpCas9-1-573-FRB-1xNLS
(Plasmid
#107298)
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PurposeExpresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107298 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 107298-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 107298-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCSDest2
- Backbone size w/o insert (bp) 4117
- Total vector size (bp) 6180
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTruncated SpCas9 (aa 1-573) fused to FRB
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SpeciesSynthetic; S. pyogenes
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Insert Size (bp)2055
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MutationTruncated SpCas9 (aa 1-573)
- Promoter CMV IE94
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Tags
/ Fusion Proteins
- SV40 NLS (C terminal on insert)
- FRB (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer Sp6
- 3′ sequencing primer T3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 107298-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 107298-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSD_N-SpCas9-1-573-FRB-1xNLS was a gift from Scot Wolfe (Addgene plasmid # 107298 ; http://n2t.net/addgene:107298 ; RRID:Addgene_107298)
