PurposeMonomeric SpyTag-6×-His alpha-hemolysin
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107334||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2745
- Total vector size (bp) 3636
Vector typeBacterial Expression
Growth in Bacteria
- Promoter T7
/ Fusion Protein
- 6xHis (C terminal on insert)
- Cloning method TOPO Cloning
- 5′ sequencing primer T7-forward
- 3′ sequencing primer T7-term-reverse (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEXP5-aHL_spytag_his was a gift from George Church (Addgene plasmid # 107334 ; http://n2t.net/addgene:107334 ; RRID:Addgene_107334)
For your References section:Design and characterization of a nanopore-coupled polymerase for single-molecule DNA sequencing by synthesis on an electrode array. Stranges PB, Palla M, Kalachikov S, Nivala J, Dorwart M, Trans A, Kumar S, Porel M, Chien M, Tao C, Morozova I, Li Z, Shi S, Aberra A, Arnold C, Yang A, Aguirre A, Harada ET, Korenblum D, Pollard J, Bhat A, Gremyachinskiy D, Bibillo A, Chen R, Davis R, Russo JJ, Fuller CW, Roever S, Ju J, Church GM. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6749-E6756. doi: 10.1073/pnas.1608271113. Epub 2016 Oct 11. 10.1073/pnas.1608271113 PubMed 27729524