WT PBRM1- TRIPZ-neo
(Plasmid #107406)

• Purpose: Mammalian expression, inducible PBRM1
• Depositing Lab: William Kaelin
• Publication: Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1027-1032. doi: 10.1073/pnas.1619726114. Epub 2017 Jan 12.

Backbone

• Vector backbone: pTRIPZ-neo
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Please grow transformed cells for 2+ days
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PBRM1
• Species: H. sapiens (human)
• Entrez Gene: PBRM1 (a.k.a. BAF180, PB1, RCC, SMARCH1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: ClaI (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is unstable and likely to recombine when grown. Upon receipt it is essential to screen multiple colonies to confirm the intact plasmid is present. Recombined products are not expected to impact viral packaging if the full length plasmid is present.