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Purposemeasure LATS activity by Luciferase Assay
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Depositing Lab
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Depositing OrganizationQueen's University
Located in Canada -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107611 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 107611-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 107611-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCDNA3.1hygro-BamHI-CLuc1433-NotI
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system.
Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind.
DNA (Catalog # 107611-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 107611-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1neo-14-3-3-CLuc was a gift from Xiaolong Yang (Addgene plasmid # 107611 ; http://n2t.net/addgene:107611 ; RRID:Addgene_107611) -
For your References section:
A LATS biosensor screen identifies VEGFR as a regulator of the Hippo pathway in angiogenesis. Azad T, Janse van Rensburg HJ, Lightbody ED, Neveu B, Champagne A, Ghaffari A, Kay VR, Hao Y, Shen H, Yeung B, Croy BA, Guan KL, Pouliot F, Zhang J, Nicol CJB, Yang X. Nat Commun. 2018 Mar 13;9(1):1061. doi: 10.1038/s41467-018-03278-w. 10.1038/s41467-018-03278-w PubMed 29535383
Map uploaded by the depositor.
