|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107736||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBong-Kiun Kaang
- Backbone size w/o insert (bp) 3372
- Total vector size (bp) 7305
Modifications to backboneA fragment containing the nEF promoter, LoxP/Lox2272, and reverse-complemented Synaptophysin-GCaMP6s.P2A.mRuby3 was swapped into replace EGFP and the shortened CamKII promoter in the CW3SL backbone. Total AAV packaging size (including ITRs and DNA in between) is 4681 bp.
Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert namesynaptophysin-GCaMP6s, mRuby3
Alt namesynaptophysin-GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
SpeciesR. norvegicus (rat); A. victoria (jellyfish)
- Promoter nEF
- Cloning method Restriction Enzyme
- 5′ cloning site Pci (not destroyed)
- 3′ cloning site SciI (destroyed during cloning)
- 5′ sequencing primer CCTGGCCTTTTGCTGGCCTTTTGC
- 3′ sequencing primer ATCCAGAGGTTGATTATCTC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byGCaMP6s was synthesized de novo based on sequences published by Douglas Kim et al, Janelia Research Campus in Chen et al, 2013 PMID: 23868258. The Synaptophysin-GCaMP6s fusion sequence was previously published by Ofer Yizhar's lab in Mahn et al, 2016 PMID: 26950004. mRuby3 sequence synthesis was based on sequences published by Jun Chu's and Michael Lin's laboratories (Bajar et al, 2016, Nature Communications, PMID:26879144)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3 was a gift from Rylan Larsen (Addgene plasmid # 107736 ; http://n2t.net/addgene:107736 ; RRID:Addgene_107736)