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(Plasmid #107767)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107767 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    NEB Biosciences
  • Backbone size w/o insert (bp) 5052
  • Total vector size (bp) 6390
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Subunit C of SDH fused to pHluorin
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    SDHC (a.k.a. CYB560, CYBL, PGL3, QPS1, SDH3)
  • Promoter CMV
  • Tag / Fusion Protein
    • pHluorin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer GFP-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SDHC-pHLuorin-sems was a gift from Karin Busch (Addgene plasmid # 107767 ; ; RRID:Addgene_107767)
  • For your References section:

    Lifetime imaging of GFP at CoxVIIIa reports respiratory supercomplex assembly in live cells. Rieger B, Shalaeva DN, Sohnel AC, Kohl W, Duwe P, Mulkidjanian AY, Busch KB. Sci Rep. 2017 Apr 6;7:46055. doi: 10.1038/srep46055. 10.1038/srep46055 PubMed 28383048