Purposeexpression of a dominant negative MAP Kinase Kinase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107974||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameMKK1 K97A
SpeciesH. sapiens (human)
Mutationdominant negative; see Depositor Comments below
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Addgene NGS results found an added alanine residue at position 2 in the protein sequence compared to the NCBI reference. This difference does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-MKK1K97A was a gift from Edwin Krebs (Addgene plasmid # 107974 ; http://n2t.net/addgene:107974 ; RRID:Addgene_107974)
For your References section:Overexpression of mitogen-activated protein kinase kinase (MAPKK) and its mutants in NIH 3T3 cells. Evidence that MAPKK involvement in cellular proliferation is regulated by phosphorylation of serine residues in its kinase subdomains VII and VIII. Seger R, Seger D, Reszka AA, Munar ES, Eldar-Finkelman H, Dobrowolska G, Jensen AM, Campbell JS, Fischer EH, Krebs EG. J Biol Chem. 1994 Oct 14;269(41):25699-709. PubMed 7929275