pSirenRetroQ(puro)-shEgr3
(Plasmid #108115)

• Purpose: pSIREN-RetroQ vector containing shRNA sequence to Egr3
• Depositing Lab: Peter Johnson
• Publication: Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. doi: 10.1128/MCB.01489-14. Epub 2014 Dec 22.

Backbone

• Vector backbone: pSirenRetroQ(puro)
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6445
• Total vector size (bp): 6504
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shRNA to Egr3
• Alt name: Early growth response 3
• gRNA/shRNA sequence: AATCAATTGCCTGACAATC
• Species: M. musculus (mouse)
• Entrez Gene: Egr3 (a.k.a. Pilot)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: U6 fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For the chosen sequence (AATCAATTGCCTGACAATC), the annealed oligos were:

5'-gatccAATCAATTGCCTGACAATCTTCAAGAGAGATTGTCAGGCAATTGATTTTTTTTACGCGTg-----3'

3'-gTTAGTTAACGGACTGTTAGAAGTTCTCTCTAACAGTCCGTTAACTAAAAAAAATGCGCActtaa-5'