UAS:bloSwitch
(Plasmid
#108355)
-
PurposeUAS driven green to red switch reporter for B3 recombinase expression
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108355 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepUC19
- Backbone size w/o insert (bp) 2700
- Total vector size (bp) 6639
-
Vector typeZebrafish expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameUAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
-
SpeciesSynthetic
-
MutationZebrafish codon optimized
- Promoter lac
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning was via SLiCE (Seamless Ligation Cloning Extract).
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
UAS:bloSwitch was a gift from Harold Burgess (Addgene plasmid # 108355 ; http://n2t.net/addgene:108355 ; RRID:Addgene_108355) -
For your References section:
Presynaptic Inhibition Selectively Gates Auditory Transmission to the Brainstem Startle Circuit. Tabor KM, Smith TS, Brown M, Bergeron SA, Briggman KL, Burgess HA. Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 10.1016/j.cub.2018.06.020. Epub 2018 Aug 2. 10.1016/j.cub.2018.06.020 PubMed 30078569
