pAJM.716
              
              
                (Plasmid
                
                #108518)
              
            
            
            
          - 
            PurposePBAD-YFP Reporter. Ara sensor for use in Marionette strain.
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              Depositing Lab
 - 
          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108518 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonep15A-Kan
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              Vector typeBacterial Expression
 
Growth in Bacteria
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            Bacterial Resistance(s)Kanamycin, 50 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)NEB Stable
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            Copy numberLow Copy
 
Gene/Insert
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                Gene/Insert namePBAD-YFP
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                    SpeciesSynthetic
 
Cloning Information
- Cloning method Gibson Cloning
 
Resource Information
- 
            
            
            Supplemental Documents
 
Terms and Licenses
- 
        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAJM.716 was a gift from Christopher Voigt (Addgene plasmid # 108518 ; http://n2t.net/addgene:108518 ; RRID:Addgene_108518) - 
                
For your References section:
Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA. Nat Chem Biol. 2018 Nov 26. pii: 10.1038/s41589-018-0168-3. doi: 10.1038/s41589-018-0168-3. 10.1038/s41589-018-0168-3 PubMed 30478458