pGS-BacA-21232
(Plasmid
#109177)
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Purpose(Empty Backbone) Plasmid for expression of C-terminally CBP-3xFLAG-ProteinA-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACEBac1
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Backbone manufacturerGeneva Biotech
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Vector typeInsect Expression
- Promoter polyhedrin
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Tag
/ Fusion Protein
- CBP-3xFLAG-ProteinA (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer polh
- 3′ sequencing primer SV40_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGS-BacA-21232 was a gift from Monika Golas (Addgene plasmid # 109177 ; http://n2t.net/addgene:109177 ; RRID:Addgene_109177) -
For your References section:
Modulating the Expression Strength of the Baculovirus/Insect Cell Expression System: A Toolbox Applied to the Human Tumor Suppressor SMARCB1/SNF5. Golas MM, Jayaprakash S, Le LTM, Zhao Z, Heras Huertas V, Jensen IS, Yuan J, Sander B. Mol Biotechnol. 2018 Nov;60(11):820-832. doi: 10.1007/s12033-018-0107-2. 10.1007/s12033-018-0107-2 PubMed 30178299
