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pGS-M1-BRG1-3xFLAG
(Plasmid #109188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109188 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACEBac2
  • Backbone manufacturer
    Geneva Biotech
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRG1
  • Alt name
    SMARCA4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4917
  • Entrez Gene
    SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
  • Promoter p10
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGS-M1-BRG1-3xFLAG was a gift from Monika Golas (Addgene plasmid # 109188 ; http://n2t.net/addgene:109188 ; RRID:Addgene_109188)

  • For your References section:

    The ATPase BRG1/SMARCA4 is a protein interaction platform that recruits BAF subunits and the transcriptional repressor REST/NRSF in neural progenitor cells. Jayaprakash S, Drakulic S, Zhao Z, Sander B, Golas MM. Mol Cell Biochem. 2019 Nov;461(1-2):171-182. doi: 10.1007/s11010-019-03600-0. Epub 2019 Aug 20. 10.1007/s11010-019-03600-0 PubMed 31428904
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