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pGS-M1-BRG1-3xFLAG-BAF57
(Plasmid #109189)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 109189 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACEBac2
  • Backbone manufacturer
    Geneva Biotech
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    BRG1
  • Alt name
    SMARCA4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4917
  • Entrez Gene
    SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
  • Promoter p10
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    BAF57
  • Alt name
    SMARCE1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SMARCE1 (a.k.a. BAF57, CSS5)
  • Promoter p10

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGS-M1-BRG1-3xFLAG-BAF57 was a gift from Monika Golas (Addgene plasmid # 109189 ; http://n2t.net/addgene:109189 ; RRID:Addgene_109189)

  • For your References section:

    The ATPase BRG1/SMARCA4 is a protein interaction platform that recruits BAF subunits and the transcriptional repressor REST/NRSF in neural progenitor cells. Jayaprakash S, Drakulic S, Zhao Z, Sander B, Golas MM. Mol Cell Biochem. 2019 Nov;461(1-2):171-182. doi: 10.1007/s11010-019-03600-0. Epub 2019 Aug 20. 10.1007/s11010-019-03600-0 PubMed 31428904
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