pGS-BacA-21222-SIN3B-3xFLAG
(Plasmid
#109214)
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PurposeEncodes human full-length SIN3B with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 109214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepGS-BacA-21222, derived from pACEBac1
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Backbone manufacturerbackbone derived from pACEBac1, Geneva Biotech
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman full-length SIN3B
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Alt nameSIN3B
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SpeciesH. sapiens (human)
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Insert Size (bp)3465
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Entrez GeneSIN3B
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer polh
- 3′ sequencing primer SV40_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGS-BacA-21222-SIN3B-3xFLAG was a gift from Monika Golas (Addgene plasmid # 109214 ; http://n2t.net/addgene:109214 ; RRID:Addgene_109214) -
For your References section:
Expression of the Neural REST/NRSF-SIN3 Transcriptional Corepressor Complex as a Target for Small-Molecule Inhibitors. Jayaprakash S, Le LTM, Sander B, Golas MM. Mol Biotechnol. 2021 Jan;63(1):53-62. doi: 10.1007/s12033-020-00283-7. Epub 2020 Nov 1. 10.1007/s12033-020-00283-7 PubMed 33130996