pKM490-Clip
(Plasmid
#109281)
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PurposeORBIT integrating plasmid for C-terminal tagging with CLIP tag
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109281 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 109281-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 109281-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepKM464
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Backbone manufacturerKenan Murphy
- Backbone size w/o insert (bp) 3082
- Total vector size (bp) 3636
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Modifications to backboneInsertion of attB-CLIP tag
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Vector typeORBIT integrating plasmid
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsGrow in LB containing 20 ug/ml chloramphenicol.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameattB-CLIP tag
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SpeciesSynthetic
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Insert Size (bp)586
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ORBIT plasmid for creating CLIP fusions to target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
DNA (Catalog # 109281-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 109281-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKM490-Clip was a gift from Kenan Murphy (Addgene plasmid # 109281 ; http://n2t.net/addgene:109281 ; RRID:Addgene_109281) -
For your References section:
ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes. Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, Sassetti CM. MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 10.1128/mBio.01467-18. 10.1128/mBio.01467-18 PubMed 30538179
Map uploaded by the depositor.
