[pSK483] pLJC5 Flag-Depdc5(P)
(Plasmid
#109342)
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PurposeLenti-viral expression of Flag-tagged Depdc5 mutant P
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLJC5
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDepdc5
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SpeciesH. sapiens (human)
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Insert Size (bp)5000
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MutationYDLLP(775-779)GSGSG
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Entrez GeneDEPDC5 (a.k.a. DEP.5, FFEVF, FFEVF1)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
[pSK483] pLJC5 Flag-Depdc5(P) was a gift from David Sabatini & Kuang Shen (Addgene plasmid # 109342 ; http://n2t.net/addgene:109342 ; RRID:Addgene_109342) -
For your References section:
Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes. Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM. Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 2018 Mar 28. 10.1038/nature26158 PubMed 29590090
