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pEM705 Gst (Cys)
(Plasmid #109376)


Item Catalog # Description Quantity Price (USD)
Plasmid 109376 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6002
  • Total vector size (bp) 7184
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Gst chimera
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Cys at position 391 (Pertussis Toxin Sensitive)
  • GenBank ID
  • Promoter CAG
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cttctggcgtgtgaccggcggctc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEM705 Gst (Cys) was a gift from Robert Lucas (Addgene plasmid # 109376 ; ; RRID:Addgene_109376)
  • For your References section:

    A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling. Ballister ER, Rodgers J, Martial F, Lucas RJ. BMC Biol. 2018 Jan 16;16(1):10. doi: 10.1186/s12915-017-0475-2. 10.1186/s12915-017-0475-2 [pii] PubMed 29338718