|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11005||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4783
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehUD recombination system
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)3047
MutationDirect repeat recombination system. Recombination reconstitutes a functional HIS3 gene
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
The repeats of HIS3 in this plasmid contain truncations of the HIS3 gene. The first repeat is a 3' end truncation that contains the promoter and the ORF from the ATG to the internal KpnI site (used for subcloning). The second repeat is a 5' truncation that contains from the internal NdeI site (used for subcloning) to the end of the gene. Recombination of the common DNA sequence between these repeats (approx. 430 nt internal to the HIS3 ORF) reconstitutes a functional gene. Both plasmids, containing the repeats in direct orientation (hUD) or inverted orientation (hUI), have been tested for recombination with positive result.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRS314-hUD was a gift from Francisco Malagon (Addgene plasmid # 11005 ; http://n2t.net/addgene:11005 ; RRID:Addgene_11005)