PurposeExpression of human eEF2K in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110160||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameeEF2K (eukaryotic elongation factor 2 kinase)
SpeciesH. sapiens (human)
Entrez GeneEEF2K (a.k.a. CaMKIII, HSU93850, eEF-2K)
- Promoter CMV
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-HA-eEF2K was a gift from Daniele Guardavaccaro (Addgene plasmid # 110160 ; http://n2t.net/addgene:110160 ; RRID:Addgene_110160)
For your References section:Coupled activation and degradation of eEF2K regulates protein synthesis in response to genotoxic stress. Kruiswijk F, Yuniati L, Magliozzi R, Low TY, Lim R, Bolder R, Mohammed S, Proud CG, Heck AJ, Pagano M, Guardavaccaro D. Sci Signal. 2012 Jun 5;5(227):ra40. doi: 10.1126/scisignal.2002718. 10.1126/scisignal.2002718 PubMed 22669845