Human USP30 (64-502, construct 2, MG-26-18)
(Plasmid
#110744)
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PurposeBacterial expression of human USP30 (construct 2)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
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Backbone manufacturerMerck
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUSP30
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SpeciesH. sapiens (human)
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Entrez GeneUSP30
- Promoter T7
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Tag
/ Fusion Protein
- N-His6-3C-GST-3C (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AATACGACTCACTATAGGG
- 3′ sequencing primer GGGTTATGCTAGTTATTGC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byUSP30 gene obtained through gene synthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human USP30 (64-502, construct 2, MG-26-18) was a gift from David Komander (Addgene plasmid # 110744 ; http://n2t.net/addgene:110744 ; RRID:Addgene_110744) -
For your References section:
Mechanism and regulation of the Lys6-selective deubiquitinase USP30. Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D. Nat Struct Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475. 10.1038/nsmb.3475 PubMed 28945249
