GAL4-mNFAT1(2-144)
(Plasmid #11108)

• Purpose: Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells
• Depositing Lab: Anjana Rao
• Publication: Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

Backbone

• Vector backbone: pM
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NFAT1
• Species: M. musculus (mouse)
• Insert Size (bp): 430
• Mutation: Contains NFAT1 amino acids 2-144; L78P and P140Q compared to GenBank reference sequence NP_035029.2
• Entrez Gene: Nfatc2 (a.k.a. AI607462, NF-ATc2, NF-ATp, NFAT1, NFAT1-D, Nfatp)
• Tag / Fusion Protein:
  • GAL4-DBD (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry