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pOEM1_pCMV:hShh-pPH:vsvged
(Plasmid #111156)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111156 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pOEM1
  • Vector type
    Mammalian Expression, Insect Expression ; Baculoviral rescue shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hShh
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1398
  • Mutation
    wt
  • Entrez Gene
    SHH (a.k.a. HHG1, HLP3, HPE3, MCOPCB5, SMMCI, ShhNC, TPT, TPTPS)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOEM1_pCMV:hShh-pPH:vsvged was a gift from Elly Tanaka (Addgene plasmid # 111156 ; http://n2t.net/addgene:111156 ; RRID:Addgene_111156)
  • For your References section:

    FGF8 and SHH substitute for anterior-posterior tissue interactions to induce limb regeneration. Nacu E, Gromberg E, Oliveira CR, Drechsel D, Tanaka EM. Nature. 2016 May 19;533(7603):407-10. doi: 10.1038/nature17972. 10.1038/nature17972 PubMed 27120163