pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE
(Plasmid
#89587)
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PurposeFor nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.
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Depositing Lab
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Depositing OrganizationNational Institute of Genetics
Located in Japan -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 89587-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 89587-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
- Backbone size w/o insert (bp) 9530
- Total vector size (bp) 10266
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenuclear localization signal (nls) turboRFP
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Alt namenlsturboRFP
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SpeciesSV40, Entacmaea quadricolor
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Insert Size (bp)736
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer EBV_rev_primer
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Backbone plasmid pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) also submitted in addgene database.
DNA (Catalog # 89587-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 89587-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE was a gift from Takuji Iwasato (Addgene plasmid # 89587 ; http://n2t.net/addgene:89587 ; RRID:Addgene_89587) -
For your References section:
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. 10.1038/srep35747 PubMed 27775045
