PurposeFor nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89587||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
- Backbone size w/o insert (bp) 9530
- Total vector size (bp) 10266
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Copy numberHigh Copy
Gene/Insert namenuclear localization signal (nls) turboRFP
SpeciesSV40, Entacmaea quadricolor
Insert Size (bp)736
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer EBV_rev_primer (Common Sequencing Primers)
Backbone plasmid pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) also submitted in addgene database.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE was a gift from Takuji Iwasato (Addgene plasmid # 89587 ; http://n2t.net/addgene:89587 ; RRID:Addgene_89587)
For your References section:Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. 10.1038/srep35747 PubMed 27775045