PurposeTERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||111375||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneThe inserted fragments of the pENTR plasmids were transferred by Gateway recombination cloning technique (Invitrogen) to lentiviral (pLenti6/BLOCK-iT-DEST and pLenti4/V5-DEST) expression vectors.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Gene/Insert nameTERRA polyA
SpeciesH. sapiens (human)
Insert Size (bp)868
- Promoter CMV
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please see the Addgene Partial NGS Result for the verified backbone. Please note this result contains only a part (around 670 bps) of the 800 bps telomere sequence repeats, with further variant repeats not seen by Sanger sequencing by the Depositor.
This plasmid may be prone to recombination due to the telomere hexanucleotide repeats, and therefore screening multiple colonies for the correct insert length is recommended before proceeding with downstream work
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV CMV-TERRA was a gift from Klaus Holzmann (Addgene plasmid # 111375 ; http://n2t.net/addgene:111375 ; RRID:Addgene_111375)
For your References section:Telomere Transcripts Target Telomerase in Human Cancer Cells. Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K. Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 10.3390/genes7080046. 10.3390/genes7080046 PubMed 27537914