PurposeTERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||111383||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneFrom the pENTR-hH1 plasmid with inserted telomere fragment, the H1 promoter was deleted for use with Gateway expression vectors with built-in expression cassettes using CMV promoters, like pAD/CMV and pLenti4/V5 (Invitrogen). To this end, the hH1 promoter was cut out with SacI and BamHI (Fermentas) and the remaining plasmid fragment was treated with Klenow fragment (Invitrogen) to generate blunt ends, ligated and transformed in competent E. coli as described before.
Vector typeGateway Entry Cloning
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)868
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Depositing Scientist Sequence may not contain all correct information about the telomere sequence repeats such as canonical and variant repeat positions, and precise information about insert length. Further, the Addgene Partial NGS Result does not include the entire region of telomere repeats. Note that the BamHI site was destroyed during cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR TERRA was a gift from Klaus Holzmann (Addgene plasmid # 111383 ; http://n2t.net/addgene:111383 ; RRID:Addgene_111383)
For your References section:Telomere Transcripts Target Telomerase in Human Cancer Cells. Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K. Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 10.3390/genes7080046. 10.3390/genes7080046 PubMed 27537914