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f.pRST1
(Plasmid #50494)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50494 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 3091
  • Modifications to backbone
    See putative plasmid sequence.
  • Vector type
    Structural DNA substrate/material

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    Telomeric inserts are unstable during prolonged culture. Recommend maintaining plasmid as a DNA stock and growing from a fresh transformation with minimal incubation times.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TTAGGG(n) Track
  • Insert Size (bp)
    136
  • Promoter T3 and T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BsmBI (not destroyed)
  • 5′ sequencing primer AAT TAA CCC TCA CTA AAG GG
  • 3′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    f.pRST1 was a gift from Jack Griffith (Addgene plasmid # 50494 ; http://n2t.net/addgene:50494 ; RRID:Addgene_50494)
  • For your References section:

    Human Rap1 interacts directly with telomeric DNA and regulates TRF2 localization at the telomere. Arat NO, Griffith JD. J Biol Chem. 2012 Dec 7;287(50):41583-94. doi: 10.1074/jbc.M112.415984. Epub 2012 Oct 20. 10.1074/jbc.M112.415984 PubMed 23086976