Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #50494)


Item Catalog # Description Quantity Price (USD)
Plasmid 50494 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 3091
  • Modifications to backbone
    See putative plasmid sequence.
  • Vector type
    Structural DNA substrate/material

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Telomeric inserts are unstable during prolonged culture. Recommend maintaining plasmid as a DNA stock and growing from a fresh transformation with minimal incubation times.
  • Copy number
    High Copy


  • Gene/Insert name
    TTAGGG(n) Track
  • Insert Size (bp)
  • Promoter T3 and T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BsmBI (not destroyed)
  • 5′ sequencing primer AAT TAA CCC TCA CTA AAG GG
  • 3′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    f.pRST1 was a gift from Jack Griffith (Addgene plasmid # 50494 ; ; RRID:Addgene_50494)
  • For your References section:

    Human Rap1 interacts directly with telomeric DNA and regulates TRF2 localization at the telomere. Arat NO, Griffith JD. J Biol Chem. 2012 Dec 7;287(50):41583-94. doi: 10.1074/jbc.M112.415984. Epub 2012 Oct 20. 10.1074/jbc.M112.415984 PubMed 23086976