pBB
(Plasmid #53210)

• Purpose: Contains a 103bp telomeric track
• Depositing Lab: Jack Griffith
• Publication: Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95. doi: 10.1021/bi5006249. Epub 2014 Aug 21.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Agilent
• Backbone size w/o insert (bp): 2948
• Total vector size (bp): 3051

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TTAGGG(n) Track
• Alt name: Telomere Track
• Alt name: T2AG3 Track
• Insert Size (bp): 103
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (not destroyed)
• 3′ cloning site: BsmBI (not destroyed)
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: AAT TAA CCC TCA CTA AAG GG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry