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pENTR U6-hTERTsh
(Plasmid #111385)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111385 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pENTR/D-hH1
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    AV expressing shRNA against human telomerase reverse transcriptase (hTERT) was constructed from retroviral vector pMKO-1P-C3 kindly provided by William C. Hahn (Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA). In brief, an expression cassette for shRNA with U6 promoter targeting region 3114–3134 from the hTERT transcript was cloned into pENTR-hH1.
  • Vector type
    Gateway Entry Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shRNA C3 against hTERT
  • gRNA/shRNA sequence
    TTTCATCAGCAAGTTTGGA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    329
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/BglII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR U6-hTERTsh was a gift from Klaus Holzmann (Addgene plasmid # 111385 ; http://n2t.net/addgene:111385 ; RRID:Addgene_111385)
  • For your References section:

    Telomere Transcripts Target Telomerase in Human Cancer Cells. Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K. Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 10.3390/genes7080046. 10.3390/genes7080046 PubMed 27537914