Agarabi CHO Light Chain in pJ201
(Plasmid
#111566)
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PurposeCloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJ201
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Backbone manufacturerATUM (formerly DNA 2.0)
- Backbone size w/o insert (bp) 2673
- Total vector size (bp) 3389
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Vector typeSynthetic Biology ; Cloning Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsCulture transformants in LB broth supplemented with 50 ug/mL Kanamycin
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMurine light chain
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SpeciesM. musculus (mouse)
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Insert Size (bp)716
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GenBank IDAKH40269.1
- Promoter None
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Agarabi CHO Light Chain in pJ201 was a gift from Cyrus Agarabi (Addgene plasmid # 111566 ; http://n2t.net/addgene:111566 ; RRID:Addgene_111566) -
For your References section:
Impact of media and antifoam selection on monoclonal antibody production and quality using a high throughput micro-bioreactor system. Velugula-Yellela SR, Williams A, Trunfio N, Hsu CJ, Chavez B, Yoon S, Agarabi C. Biotechnol Prog. 2018 Jan;34(1):262-270. doi: 10.1002/btpr.2575. Epub 2017 Nov 16. 10.1002/btpr.2575 PubMed 29086492
Map uploaded by the depositor.
