pFLAG rat Antizyme 1
(Plasmid #11183)

• Depositing Lab: Bruce Zetter
• Publication: Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-11.

Backbone

• Vector backbone: pFLAG
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AZ1
• Alt name: ornithine decarboxylase antizyme 1
• Alt name: antizyme 1
• Alt name: OAZ1
• Species: R. norvegicus (rat)
• Insert Size (bp): 700
• GenBank ID: NM_139081
• Entrez Gene: Oaz1 (a.k.a. ODC-Az, ODCAC, Oaz)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry