pCRII-TOPO-ZFERV
(Plasmid #11192)

• Depositing Lab: Lisa Steiner
• Publication: Shen et al J Virol. 2004 Jan . 78(2):899-911.

Backbone

• Vector backbone: pCRII-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3950
• Vector type: Zebrafish
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZFERV
• Species: D. rerio (zebrafish)
• Insert Size (bp): 11249
• GenBank ID: AF503912
• Entrez Gene: erv (a.k.a. ZFERV, si:dkey-266f7.7, wu:fd52b06, wu:fi23c07, zmp:0000000535)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ cloning site: TOPO TA cloned (not destroyed)
• 3′ cloning site: TOPO TA cloned (not destroyed)
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ZFERV PCR insert (the single 3' A overhangs on both ends are not included): 5'-TCTAAAGGAAAATGAACTTAACAGTTGCGAG (31 nt total upstream of the ZFERV sequence), CGAGgacaac agtggaatgg aatctttgtt aaatttagca
aagtacagta aactgaacag agtattgcga ata -3'
(73 nt total downstream of the ZFERV sequence).
A map of pCRII-TOPO is available at Addgene's vector DB.