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pNAS1b split mCherry NEDD4 WW2 neg ctrl
(Plasmid #112030)


Item Catalog # Description Quantity Price (USD)
Plasmid 112030 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
  • Tag / Fusion Protein
    • Split mCherry

Resource Information

Depositor Comments

Backbone originally described in Sawyer, et al, Designed Phosphoprotein Recognition in Escherichia coli, 2014.

Binding domain under araBAD, phosphosite/peptide cassette under pLtetO

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNAS1b split mCherry NEDD4 WW2 neg ctrl was a gift from Jesse Rinehart (Addgene plasmid # 112030 ; ; RRID:Addgene_112030)
  • For your References section:

    Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J. Nat Biotechnol. 2018 Aug;36(7):638-644. doi: 10.1038/nbt.4150. Epub 2018 Jun 11. 10.1038/nbt.4150 PubMed 29889213