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Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions.
Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J
Nat Biotechnol. 2018 Aug;36(7):638-644. doi: 10.1038/nbt.4150. Epub 2018 Jun 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
111880pNAS1b split mCherry 14-3-3βMode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)
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111881pNAS1b split mCherry 14-3-3σMode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)
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111882pNAS1b split mCherry NEDD4 WW2Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)
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111883pNAS1b split mCherry NEDD4-2 WW2Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)
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112029pNAS1b split mCherry NEDD4 WW2 pos ctrlMode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
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112030pNAS1b split mCherry NEDD4 WW2 neg ctrlMode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)
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112031pNAS1b split mCherry 14-3-3β ctrlMode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
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