pNAS1b split mCherry 14-3-3β ctrl
PurposeMode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||112031||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeBacterial Expression
Growth in Bacteria
Gene/Insert name14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
/ Fusion Protein
- Split mCherry
Terms and Licenses
- Not Available to Industry
Backbone originally described in Sawyer, et al, Designed Phosphoprotein Recognition in Escherichia coli, 2014.
Binding domain under araBAD, phosphosite/peptide cassette under pLtetO
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNAS1b split mCherry 14-3-3β ctrl was a gift from Jesse Rinehart (Addgene plasmid # 112031 ; http://n2t.net/addgene:112031 ; RRID:Addgene_112031)
For your References section:Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J. Nat Biotechnol. 2018 Aug;36(7):638-644. doi: 10.1038/nbt.4150. Epub 2018 Jun 11. 10.1038/nbt.4150 PubMed 29889213