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pNAS1b split mCherry 14-3-3β
(Plasmid #111880)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111880 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNAS1b
  • Backbone size (bp) 5500
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • Split mCherry system: 14-3-3β fused to C-terminal split mCherry; phosphosite empty cassette fused to N-terminal split mCherry. (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Depositor Comments

Backbone originally described in Sawyer, et al, Designed Phosphoprotein Recognition in Escherichia coli, 2014.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNAS1b split mCherry 14-3-3β was a gift from Jesse Rinehart (Addgene plasmid # 111880 ; http://n2t.net/addgene:111880 ; RRID:Addgene_111880)
  • For your References section:

    Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J. Nat Biotechnol. 2018 Aug;36(7):638-644. doi: 10.1038/nbt.4150. Epub 2018 Jun 11. 10.1038/nbt.4150 PubMed 29889213