pBABE Rad C249
(Plasmid #11215)

• Depositing Lab: Ronald Kahn
• Publication: Moyers et al J Biol Chem. 1997 May 2. 272(18):11832-9.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Rad
• Species: H. sapiens (human)
• Insert Size (bp): 800
• Mutation: C249: deletion of amino acids 248-308
• Entrez Gene: RRAD (a.k.a. RAD, RAD1, REM3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The C249 mutant contains a deletion of the calmodulin binding site and all C-terminal in vitro phosphorylation sites of Rad. Localizes mainly to cytosol in C2C12 cells (displaced from membrane).