pBABE Rad N88
(Plasmid #11218)

• Depositing Lab: Ronald Kahn
• Publication: Moyers et al J Biol Chem. 1997 May 2. 272(18):11832-9.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rad
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: N88: deletion of amino acids 1-88
• Entrez Gene: RRAD (a.k.a. RAD, RAD1, REM3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The N88 mutant is localized to the cytoskeletal and membrane fractions of C2C12 cells (displaced from cytosol) and exhibits enhanced calmodulin binding relative to wildtype Rad.