pGW1-Myc-Sarm1
(Plasmid #50707)

• Purpose: expression of Sarm1 in mammalian cells
• Depositing Lab: Yi-Ping Hsueh
• Publication: Chen et al J Cell Biol. 2011 May 16;193(4):769-84. doi: 10.1083/jcb.201008050. Epub 2011 May 9.

Backbone

• Vector backbone: pGW1-CMV-Myc
• Backbone size w/o insert (bp): 5371
• Modifications to backbone: Myc tag added to pGW1-CMV
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sarm1
• Alt name: sterile alpha and HEAT/Armadillo motif containing 1
• Species: M. musculus (mouse)
• Insert Size (bp): 2172
• GenBank ID: NM_172795
• Entrez Gene: Sarm1 (a.k.a. A830091I15Rik, C78606, MyD885, Sarm)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ctgagcagtactcgttgc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mouse Sarm1 IMAGE clone was ordered from Invitrogen and further subcloned into the vector pGW1-CMV with a Myc tag. The final product pGW1-Myc-Sarm1 is going to deposit to Addgene.

There is a 1bp insertion in Addgene's quality control sequence when compared to the depositor's reference sequence. The insertion does not affect epitope tag or ORF.