-
Purposeexpression of Sarm1 in mammalian cells
-
Depositing Lab
-
Depositing OrganizationAcademia Sinica
Located in Taiwan -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 50707-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 50707-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepGW1-CMV-Myc
- Backbone size w/o insert (bp) 5371
-
Modifications to backboneMyc tag added to pGW1-CMV
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSarm1
-
Alt namesterile alpha and HEAT/Armadillo motif containing 1
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)2172
-
GenBank IDNM_172795
-
Entrez GeneSarm1 (a.k.a. A830091I15Rik, C78606, MyD885, Sarm)
- Promoter CMV
-
Tag
/ Fusion Protein
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer ctgagcagtactcgttgc
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The mouse Sarm1 IMAGE clone was ordered from Invitrogen and further subcloned into the vector pGW1-CMV with a Myc tag. The final product pGW1-Myc-Sarm1 is going to deposit to Addgene.
There is a 1bp insertion in Addgene's quality control sequence when compared to the depositor's reference sequence. The insertion does not affect epitope tag or ORF.
DNA (Catalog # 50707-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 50707-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGW1-Myc-Sarm1 was a gift from Yi-Ping Hsueh (Addgene plasmid # 50707 ; http://n2t.net/addgene:50707 ; RRID:Addgene_50707) -
For your References section:
Sarm1, a negative regulator of innate immunity, interacts with syndecan-2 and regulates neuronal morphology. Chen CY, Lin CW, Chang CY, Jiang ST, Hsueh YP. J Cell Biol. 2011 May 16;193(4):769-84. doi: 10.1083/jcb.201008050. Epub 2011 May 9. 10.1083/jcb.201008050 PubMed 21555464
Map uploaded by the depositor.
