SAP145
(Plasmid #11239)

• Depositing Lab: Robin Reed
• Publication: Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Backbone

• Vector backbone: pBS SK+
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SAP145
• Species: H. sapiens (human)
• Insert Size (bp): 2840
• Mutation: First 7 amino acids are missing compared to NP_006833.2
• Entrez Gene: SF3B2 (a.k.a. CFM, Cus1, HFM, SAP145, SF3B145, SF3b1, SF3b150)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reed lab clone #130.
Addgene Comment: please note that the amino acid sequence differs from the predicted sequence in Fig 1 of the publication. The SAP145 sequence matches amino acids 8-895 compared to NCBI reference: NP_006833.2.