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pExp-DsbC
(Plasmid #112564)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112564 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pOP2S
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • 8xHis-DsbC (N terminal on backbone)
    • Strep tag (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • 8xHis-DsbC (N terminal on backbone)
    • Strep tag (C terminal on backbone)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Contains N-terminal 8xHis, fusion partner: DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli, UniProtKB: P0AEG6, PMID: 23607455) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pExp-DsbC was a gift from Marko Hyvönen (Addgene plasmid # 112564 ; http://n2t.net/addgene:112564 ; RRID:Addgene_112564)