Marko Hyvönen Lab:
Protein expressionists toolkit
Lulla A, Hyvönen M
unpublished
Lulla A, Hyvönen M
unpublished
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 112558 | pExp-NHis | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag |
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| 112559 | pExp-CHis | Used for expression of protein in E. coli as C-terminual 8His fusions |
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| 112560 | pExp-AFV | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tag |
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| 112561 | pExp-Bla | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag |
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| 112562 | pExp-DsbA | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag |
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| 112563 | pExp-DsbA(SS) | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag |
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| 112564 | pExp-DsbC | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag |
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| 112565 | pExp-GB1 | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag |
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| 112566 | pExp-GST | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag |
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| 112567 | pExp-IF2 | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag |
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| 112568 | pExp-MBP | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag |
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| 112569 | pExp-mCherry | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag |
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| 112570 | pExp-MsyB | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag |
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| 112571 | pExp-NusA | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag |
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| 112572 | pExp-PpiB | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag |
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| 112573 | pExp-sfGFP | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag |
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| 112574 | pExp-Sumo | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag |
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| 112575 | pExp-TF | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag |
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| 112576 | pExp-TFSumo | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tag |
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| 112577 | pExp-Trx | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag |
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| 112578 | pExp-Tsf | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tag |
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| 129237 | pExp-Lipo | Protein expression in E.coli |
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| 129238 | pExp-Calm | Protein expression in E.coli |
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| 129239 | pExp-LSL | Protein expression in E.coli |
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| 129240 | pExp-Spy | Protein expression in E.coli |
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| 129241 | pExp-Skp | Protein expression in E.coli |
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| 129243 | pExp-DsbC(SS) | Protein expression in E.coli |
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| 129245 | pExp-Stag | Protein expression in E.coli |
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| 129246 | pExp-T7tag | Protein expression in E.coli |
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| 129247 | pExp-Etag | Protein expression in E.coli |
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