Marko Hyvönen Lab:
Protein expressionists toolkit
Lulla A, Hyvönen M
unpublished
Lulla A, Hyvönen M
unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112558 | pExp-NHis | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag |
| 112559 | pExp-CHis | Used for expression of protein in E. coli as C-terminual 8His fusions |
| 112560 | pExp-AFV | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tag |
| 112561 | pExp-Bla | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag |
| 112562 | pExp-DsbA | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag |
| 112563 | pExp-DsbA(SS) | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag |
| 112564 | pExp-DsbC | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag |
| 112565 | pExp-GB1 | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag |
| 112566 | pExp-GST | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag |
| 112567 | pExp-IF2 | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag |
| 112568 | pExp-MBP | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag |
| 112569 | pExp-mCherry | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag |
| 112570 | pExp-MsyB | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag |
| 112571 | pExp-NusA | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag |
| 112572 | pExp-PpiB | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag |
| 112573 | pExp-sfGFP | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag |
| 112574 | pExp-Sumo | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag |
| 112575 | pExp-TF | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag |
| 112576 | pExp-TFSumo | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tag |
| 112577 | pExp-Trx | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag |
| 112578 | pExp-Tsf | Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tag |
| 129237 | pExp-Lipo | Protein expression in E.coli |
| 129238 | pExp-Calm | Protein expression in E.coli |
| 129239 | pExp-LSL | Protein expression in E.coli |
| 129240 | pExp-Spy | Protein expression in E.coli |
| 129241 | pExp-Skp | Protein expression in E.coli |
| 129243 | pExp-DsbC(SS) | Protein expression in E.coli |
| 129245 | pExp-Stag | Protein expression in E.coli |
| 129246 | pExp-T7tag | Protein expression in E.coli |
| 129247 | pExp-Etag | Protein expression in E.coli |