|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||129237||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Modifications to backboneContains N-terminal 8xHis, fusion partner: Lipo (Lipoyl-binding domain of Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex from Geobacillus stearothermophilus, UniProtKB: P11961) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII.
Vector typeBacterial Expression
- Promoter T7
/ Fusion Proteins
- Strep (C terminal on backbone)
- His (N terminal on insert)
Growth in Bacteria
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pExp-Lipo was a gift from Marko Hyvönen (Addgene plasmid # 129237 ; http://n2t.net/addgene:129237 ; RRID:Addgene_129237)