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(Plasmid #84650)


Item Catalog # Description Quantity Price (USD)
Plasmid 84650 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    EMD Chemicals
  • Modifications to backbone
    malE expression cassette of pMAL-c2 (New England Biolabs) was modified for Gateway cloning. The region containing the lacIq gene and modified malE expression cassette was then subcloned into the pRARE plasmid.
  • Vector type
    Bacterial Expression
  • Promoter tac
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRARE-MBP-DEST was a gift from Neil Olszewski (Addgene plasmid # 84650 ; ; RRID:Addgene_84650)
  • For your References section:

    The Arabidopsis O-linked N-acetylglucosamine transferase SPINDLY interacts with class I TCPs to facilitate cytokinin responses in leaves and flowers. Steiner E, Efroni I, Gopalraj M, Saathoff K, Tseng TS, Kieffer M, Eshed Y, Olszewski N, Weiss D. Plant Cell. 2012 Jan;24(1):96-108. doi: 10.1105/tpc.111.093518. Epub 2012 Jan 20. 10.1105/tpc.111.093518 PubMed 22267487