|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11261||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescriptII SK+
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)2300
Entrez Geneikzf1 (a.k.a. ikaros, znfn1a1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
To make probes: antisense SalI - T3, sense XbaI - T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Ikaros was a gift from Lisa Steiner (Addgene plasmid # 11261 ; http://n2t.net/addgene:11261 ; RRID:Addgene_11261)
For your References section:Ikaros expression as a marker for lymphoid progenitors during zebrafish development. Willett CE, Kawasaki H, Amemiya CT, Lin S, Steiner LA. Dev Dyn. 2001 Dec . 222(4):694-8. 10.1002/dvdy.1223 PubMed 11748838