PurposeExpression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113037||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 5716
Vector typeMammalian Expression, CRISPR, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSV40 NLS-AcrIIA4
Addgene QC sequence analysis identified a mutation within NeoR/KanR, which results in an early stop codon. The effect of this mutation has not been determined experimentally. Ampicillin should be used for selection in bacteria.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CMV-NLS-AcrIIA4 was a gift from Dominik Niopek (Addgene plasmid # 113037 ; http://n2t.net/addgene:113037 ; RRID:Addgene_113037)
For your References section:Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Borner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D. Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9. 10.1038/s41592-018-0178-9 PubMed 30377362