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Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9.
Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Borner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D
Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113033CMV-SpyCas9SpyCas9 expression in mammalian cells (CMV promoter driven)
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113034pAAV-CMV-SpyCas9AAV vector for expression of SpyCas9 in mammalian cells
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113035CMV-CASANOVACASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
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113036pAAV-CMV-CASANOVAAAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
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113037CMV-NLS-AcrIIA4Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)
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113038pAAV-CMV-NLS-AcrIIA4AAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)
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113039pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)
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113040pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)
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113041pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)
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113042pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold) AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)
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113129dSpyCas9-p300-P2A-CASANOVACo-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA
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113130IL1RN gRNA1Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
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113131IL1RN gRNA2Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
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113132IL1RN gRNA3Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
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113133IL1RN gRNA4Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
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