Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9.
Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Borner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D
Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9. PubMed Article
Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Borner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D
Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113033 | CMV-SpyCas9 | SpyCas9 expression in mammalian cells (CMV promoter driven) |
| 113034 | pAAV-CMV-SpyCas9 | AAV vector for expression of SpyCas9 in mammalian cells |
| 113035 | CMV-CASANOVA | CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9 |
| 113036 | pAAV-CMV-CASANOVA | AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9 |
| 113037 | CMV-NLS-AcrIIA4 | Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven) |
| 113038 | pAAV-CMV-NLS-AcrIIA4 | AAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven) |
| 113039 | pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9) | AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9) |
| 113040 | pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold) | AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold) |
| 113041 | pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold) | AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold) |
| 113042 | pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold) | AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold) |
| 113129 | dSpyCas9-p300-P2A-CASANOVA | Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA |
| 113130 | IL1RN gRNA1 | Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold) |
| 113131 | IL1RN gRNA2 | Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold) |
| 113132 | IL1RN gRNA3 | Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold) |
| 113133 | IL1RN gRNA4 | Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold) |