Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #113130)


Item Catalog # Description Quantity Price (USD)
Plasmid 113130 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    scAAV vector
  • Total vector size (bp) 5283
  • Vector type
    Mammalian Expression, AAV, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
  • Promoter U6 for gRNA expression, RSV for GFP expression

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer n.d.
  • 3′ sequencing primer n.d.
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Addgene Next-Generation Sequence analysis identified numerous sequence discrepancies in ITR sequences. Plasmid is expected to function as described in the associated publication: PMID: 30377362

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IL1RN gRNA1 was a gift from Dominik Niopek (Addgene plasmid # 113130 ; ; RRID:Addgene_113130)
  • For your References section:

    Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Borner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D. Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9. 10.1038/s41592-018-0178-9 PubMed 30377362