PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113130||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonescAAV vector
- Total vector size (bp) 5283
Vector typeMammalian Expression, AAV, CRISPR, Synthetic Biology
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameIL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
- Promoter U6 for gRNA expression, RSV for GFP expression
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
- 3′ cloning site BbsI (destroyed during cloning)
- 5′ sequencing primer n.d.
- 3′ sequencing primer n.d. (Common Sequencing Primers)
Terms and Licenses
Addgene Next-Generation Sequence analysis identified numerous sequence discrepancies in ITR sequences. Plasmid is expected to function as described in the associated publication: PMID: 30377362
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:IL1RN gRNA1 was a gift from Dominik Niopek (Addgene plasmid # 113130 ; http://n2t.net/addgene:113130 ; RRID:Addgene_113130)
For your References section:Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Borner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D. Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9. 10.1038/s41592-018-0178-9 PubMed 30377362