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T777T
(Plasmid #113082)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113082 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    T444T
  • Modifications to backbone
    We further improved the T444T RNAi vector by incorporating a lacZ coding region and its regulator sequences (promoter and terminator), together with two Cas9/gRNA target sites, between the two T7 promoters of T444T.
  • Vector type
    Bacterial Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For RNAi induction in C. elegans use HT115 as growth strain.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

For plasmid usage, please see the associated publication: Sturm et al.
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Nucleic Acids Research, gky516, https://doi.org/10.1093/nar/gky516

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T777T was a gift from Tibor Vellai (Addgene plasmid # 113082 ; http://n2t.net/addgene:113082 ; RRID:Addgene_113082)
  • For your References section:

    Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T. Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516. 10.1093/nar/gky516 PubMed 29924347