T777T
(Plasmid #113082)

• Purpose: (Empty Backbone) Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
• Depositing Lab: Tibor Vellai
• Publication: Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516.

Backbone

• Vector backbone: T444T
• Modifications to backbone: We further improved the T444T RNAi vector by incorporating a lacZ coding region and its regulator sequences (promoter and terminator), together with two Cas9/gRNA target sites, between the two T7 promoters of T444T.
• Vector type: Bacterial Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For RNAi induction in C. elegans use HT115 as growth strain.
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For plasmid usage, please see the associated publication: Sturm et al.
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Nucleic Acids Research, gky516, https://doi.org/10.1093/nar/gky516