Purpose(Empty Backbone) Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113082||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneWe further improved the T444T RNAi vector by incorporating a lacZ coding region and its regulator sequences (promoter and terminator), together with two Cas9/gRNA target sites, between the two T7 promoters of T444T.
Vector typeBacterial Expression, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsFor RNAi induction in C. elegans use HT115 as growth strain.
Copy numberHigh Copy
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
For plasmid usage, please see the associated publication: Sturm et al.
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Nucleic Acids Research, gky516, https://doi.org/10.1093/nar/gky516
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:T777T was a gift from Tibor Vellai (Addgene plasmid # 113082 ; http://n2t.net/addgene:113082 ; RRID:Addgene_113082)
For your References section:Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T. Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516. 10.1093/nar/gky516 PubMed 29924347