Purpose(Empty Backbone) Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113083||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepDD162 (Peft-3::Cas9 + Empty sgRNA, Addgene no.: #47549)
Modifications to backboneThe original C. elegans Cas9 vector does not include any restriction enzyme cleavage site between the U6 promoter and gRNA sequence that would allow the spacer sequence to be efficiently modified. The redesigned vector (Ccas9lacZ) contains two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences which allow the spacer sequence to be easily modified with restriction digestion coupled linker ligation and homologous recombination-based cloning too. The new vector also contains a lacZ gene that allows blue-white selection after cloning.
Vector typeWorm Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
For plasmid usage, please see the associated publication: Sturm et al.
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Nucleic Acids Research, gky516, https://doi.org/10.1093/nar/gky516
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Ccas9LacZ was a gift from Tibor Vellai (Addgene plasmid # 113083 ; http://n2t.net/addgene:113083 ; RRID:Addgene_113083)
For your References section:Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T. Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516. 10.1093/nar/gky516 PubMed 29924347