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Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research.
Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T
Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113081T444TEnhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.
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113082T777TEnhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
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113083Ccas9LacZEnhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.
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